Improved method for bacterial cell capture after flow cytometry cell sorting.

نویسندگان

  • D Guillebault
  • M Laghdass
  • P Catala
  • I Obernosterer
  • P Lebaron
چکیده

Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 76 21  شماره 

صفحات  -

تاریخ انتشار 2010